IL-7 Protein (AA 26-177) (His tag)

Catalog No. ABIN1691666

This Recombinant IL-7 protein is produced in Human Cells.

Quick Overview for IL-7 Protein (AA 26-177) (His tag) (ABIN1691666)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 26-177

Purification tag / Conjugate: This IL-7 protein is labelled with His tag.

Purpose: Recombinant Human Interleukin-7/IL-7 (C-6His)

Sequence: DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EHVDHHHHHH

Characteristics:

Recombinant Human Interleukin-7/IL-7 (C-6His)

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: Interleukin-7/IL-7

Background: Recombinant Human Interleukin-7/IL-7 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp26-His177) of Human IL-7 fused with a polyhistidine tag at the C-terminus.
Human Interleukin 7 (IL-7) is a potent lymphoid cell growth factor stimulating the proliferation of lymphoid progenitors. IL7 can associate with the hepatocyte growth factor (HGF) to form a hybrid cytokine that functions as a pre-pro-B cell growth-stimulating factor. Human IL7 cDNA encodes a 177 AA precursor protein containing a 25 AA signal peptide and a 152 AA mature protein. Human and mouse IL7 share 65 % sequence identity in the mature region and both exhibit cross-species activity. IL-7 signals via IL-7 receptor (IL7R) activating multiple pathways including JaK/STAT and PI3K/AKT, which regulate lymphocyte survival, glucose uptake, proliferation, and differentiation. IL-7 is also associated with cytoplasmic IL2-R γ for signal transduction.

Molecular Weight: 18.4 kDa

UniProt: P13232

Pathways: JAK-STAT Signaling