ATG10 Protein (AA 1-190) (T7 tag,His tag)
Quick Overview for ATG10 Protein (AA 1-190) (T7 tag,His tag) (ABIN1691826)
Target
See all ATG10 ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 1-190
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Purification tag / Conjugate
- This ATG10 protein is labelled with T7 tag,His tag.
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Purpose
- Recombinant Human Autophagy Related 10 Homolog/ATG10 (C-6His, N-T7 tag)
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Sequence
- MASMTGGQQM GRGSMEEDEF IGEKTFQRYC AEFIKHSQQI GDSWEWRPSK DCSDGYMCKI HFQIKNGSVM SHLGASTHGQ TCLPMEEAFE LPLDDCEVIE TAAASEVIKY EYHVLYSCSY QVPVLYFRAS FLDGRPLTLK DIWEGVHECY KMRLLQGPWD TITQQEHPIL GQPFFVLHPC KTNEFMTPVL KNSQKINKNV NYITLEHHHH HH
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Characteristics
- Recombinant Human Autophagy Related 10 Homolog is produced by our E.coli expression system and the target gene encoding Met1-Thr190 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Autophagy Related 10 (ATG10) (AA 1-215) protein (His tag)
custom-made ATG10 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Autophagy Related 10 (ATG10) (AA 1-220) protein (His tag)custom-made ATG10 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Autophagy Related 10 (ATG10) (AA 1-220) protein (GST tag)ATG10 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Autophagy Related 10 (ATG10) (AA 1-220) protein (His tag)ATG10 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Autophagy Related 10 (ATG10) (AA 1-220) protein (His-IF2DI Tag)ATG10 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Supplied as a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 8.0.
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Handling Advice
- minimize freeze-thaw cycles
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Storage
- -20 °C
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Storage Comment
- Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
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Expiry Date
- 6 months
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- ATG10 (Autophagy Related 10 (ATG10))
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Alternative Name
- Ubiquitin-Like-Conjugating Enzyme ATG10/ATG10
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Background
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Alternative Names: Ubiquitin-Like-Conjugating Enzyme ATG10, Autophagy-Related Protein 10, APG10-Like, ATG10Recombinant Human Ubiquitin-Like-Conjugating Enzyme ATG10/ATG10 produced by E. coli expression system. The target protein is expressed with sequence (Met1-Thr190) of Human ATG10 fused with a 6His tag at the C-terminus.
Ubiquitin-Like-Conjugating Enzyme ATG10 (ATG10) is a ubiquitous 28 kDa member of the ATG10 family protein. ATG10 acts as an E2-like enzyme, catalyzes the transfer of ATG12 to ATG5 during in the initial stages of autophagesome formation. The heterodimer of ATG5 and ATG12 subsequntly associates non-covalently with an ATG16 multimer to generate an antophagosome. ATG10 plays a role in the conjugation of ATG12 to ATG5 by interaction with MAP1LC3A. In addition, ATG10 can diretly interact with ATG5 or ATG7. -
Molecular Weight
- 24.5 kDa
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UniProt
- Q9H0Y0
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Pathways
- ER-Nucleus Signaling, Autophagy
Target
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