CA3 Protein (AA 2-260) (His tag)
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- Target See all CA3 Proteins
- CA3 (Carbonic Anhydrase III (CA3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 2-260
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This CA3 protein is labelled with His tag.
- Purpose
- Recombinant Human Carbonic Anhydrase 3/CA3 (C-6His)
- Sequence
- AKEWGYASHN GPDHWHELFP NAKGENQSPI ELHTKDIRHD PSLQPWSVSY DGGSAKTILN NGKTCRVVFD DTYDRSMLRG GPLPGPYRLR QFHLHWGSSD DHGSEHTVDG VKYAAELHLV HWNPKYNTFK EALKQRDGIA VIGIFLKIGH ENGEFQIFLD ALDKIKTKGK EAPFTKFDPS CLFPACRDYW TYQGSFTTPP CEECIVWLLL KEPMTVSSDQ MAKLRSLLSS AENEPPVPLV SNWRPPQPIN NRVVRASFKL EHHHHHH
- Characteristics
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Recombinant Human Carbonic Anhydrase 3/CA3 (C-6His)
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
- Top Product
- Discover our top product CA3 Protein
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Carbonic Anhydrase III (CA3) (AA 1-260) protein (GST tag)
CA3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase III (CA3) (AA 2-260) protein (His tag)Crystallography grade CA3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase III (CA3) (AA 2-260) protein (His tag)Crystallography grade CA3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase III (CA3) protein (Myc-DYKDDDDK Tag)CA3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Liquid
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Supplied as a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- -80 °C
- Storage Comment
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Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles. - Expiry Date
- 6 months
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- Target
- CA3 (Carbonic Anhydrase III (CA3))
- Alternative Name
- Carbonic Anhydrase 3/CA3 (CA3 Products)
- Synonyms
- CAIII Protein, Car3 Protein, BB219044 Protein, Ca3 Protein, Car-3 Protein, carbonic anhydrase 3 Protein, CA3 Protein, CAH3 Protein, Car3 Protein, Ca3 Protein
- Background
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Recombinant Human Carbonic Anhydrase 3/CA3 is produced with our E. coli expression system. The target protein is expressed with sequence (Ala2-Lys260) of Human CA3 fused with a 6His tag at the C-terminus.
Carbonic Anhydrase 3 (CA3) belongs to the α-Carbonic Anhydrase family that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and it is present at high levels in skeletal muscle with much lower levels found in cardiac and smooth muscle. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. CA3 is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. - Molecular Weight
- 30.6 kDa
- UniProt
- P07451
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