MIF Protein (AA 2-115) (His tag)

Catalog No. ABIN1880602

Product Details

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Protein Type: Recombinant

Protein Characteristics: AA 2-115

Origin: Mouse

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MIF protein is labelled with His tag.

Purpose: Recombinant Mouse Macrophage Migration Inhibitory Factor/MIF (C-6His)

Sequence: PMFIVNTNVP RASVPEGFLS ELTQQLAQAT GKPAQYIAVH VVPDQLMTFS GTNDPCALCS LHSIGKIGGA QNRNYSKLLC GLLSDRLHIS PDRVYINYYD MNAANVGWNG STFALEHHHH HH

Characteristics: Recombinant Mouse Macrophage migration inhibitory factor/MIF is produced with our E. coli expression system. The target protein is expressed with sequence (Pro2-Ala115) of Mouse MIF fused with a polyhistidine tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: Macrophage Migration Inhibitory Factor/MIF (MIF Products)

Synonyms: mif Protein, Mif Protein, gif Protein, glif Protein, mmif Protein, LOC100136498 Protein, LOC100284350 Protein, LOC100284546 Protein, GIF Protein, Glif Protein, GLIF Protein, MMIF Protein, macrophage migration inhibitory factor L homeolog Protein, macrophage migration inhibitory factor Protein, macrophage migration inhibitory factor (glycosylation-inhibiting factor) Protein, mif.L Protein, mif Protein, MIF Protein, Mif Protein, PHATRDRAFT_49660 Protein, LOC100136498 Protein, cl405_1 Protein, LOC100284546 Protein

Background: Macrophage migration inhibitory factor(MIF) is a secreted protein and belongs to the MIF family. MIF is an important regulator of innate immunity. The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. Hence MIF is classified as an inflammatory cytokine. Furthermore glucocorticoids also stimulate white blood cells to release MIF and hence MIF partially counter acts the inhibitory effects that glucocorticoids have on the immune system. Finally trauma activates the anterior pituitary gland to release MIF.

Molecular Weight: 13.5 kDa

UniProt: P34884

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling