EGF Protein (AA 977-1029)
Quick Overview for EGF Protein (AA 977-1029) (ABIN2017757)
Target
See all EGF ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 977-1029
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Characteristics
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ED50 < 0.1 ng/mL, measured by a cell proliferation assay using BALB/c 3T3 cells, corresponding to a specific activity of > 1.0x 10^7 units/mg.
AA 977-1029, expressed with an N-terminal Met. -
Endotoxin Level
- < 0.2 EU/μg, determined by LAL method.
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Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein
EGF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Human Host: Escherichia coli (E. coli) > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (hFc Tag)EGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (His tag)EGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, AcA Active
Epidermal Growth Factor (EGF) (AA 974-1026) (Active) protein (His tag)EGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) proteinEGF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA Active
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (Fc Tag)EGF Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstituted in ddH2O at 100 μg/mL.
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Buffer
- Lyophilized after extensive dialysis against PBS.
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Storage
- -80 °C
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Storage Comment
- Lyophilized recombinant mouse Epidermal Growth Factor (rmEGF) remains stable up to 6 months at -80 °C from date of receipt. Upon reconstitution, rmEGF should be stable up to 2 weeks at 4 °C or up to 3 months at -20 °C.
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Expiry Date
- 6 months
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- EGF (Epidermal Growth Factor (EGF))
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Background
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Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor).Recombinant mouse Epidermal Growth Factor (rmEGF) produced in E. coli is a single non-glycosylated polypeptide chain containing 54 amino acids. A fully biologically active molecule, rmEGF is obtained by proprietary chromatographic techniques with a molecular mass of 6.2 kDa analyzed by reducing SDS-PAGE.
Synonyms: Epidermal Growth Factor, Urogastrone, URG -
Molecular Weight
- 6.2 kDa, observed by reducing SDS-PAGE.
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UniProt
- P01132
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Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Target
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