TNF alpha Protein (AA 84-235)
(1 reference)Quick Overview for TNF alpha Protein (AA 84-235) (ABIN2018408)
Target
See all TNF alpha ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 84-235
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Characteristics
- ED50<0.01 ng/mL, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of >1 x 108 units/mg.
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Endotoxin Level
- < 1 EU/μg, determined by LAL method.
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Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag,Biotin)
TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His-Avi Tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) protein (His tag)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) protein (His tag)custom-made TNF alpha Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor alpha (TNF alpha) (AA 1-235) protein (His tag)custom-made TNF alpha Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) proteinHPLC verified TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) (Active) protein (His tag,AVI tag,Biotin)TNF alpha Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha Origin: Rabbit Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Tumor Necrosis Factor alpha (TNF alpha) (Active) proteinTNF alpha Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstituted in ddH2O or PBS at 100 μg/mL.
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Buffer
- Lyophilized after extensive dialysis against PBS.
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Storage
- -80 °C
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Storage Comment
- Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmTNF-alpha should be stable up to 1 week at 4°C or up to 2 months at -20°C.
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Expiry Date
- 6 months
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: "Bacterial effector binding to ribosomal protein s3 subverts NF-kappaB function." in: PLoS pathogens, Vol. 5, Issue 12, pp. e1000708, (2009) (PubMed).
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: "Bacterial effector binding to ribosomal protein s3 subverts NF-kappaB function." in: PLoS pathogens, Vol. 5, Issue 12, pp. e1000708, (2009) (PubMed).
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- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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Alternative Name
- TNF-alpha
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Background
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Sharing 79 % sequence identity with human Tumor Necrosis Factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells [1]. A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form [2]. The trimeric active TNF-α[3] then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 [4]. Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
Synonyms: TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2 -
Molecular Weight
- 17kDa, observed by reducing SDS-PAGE.
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UniProt
- P06804
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Pathways
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
Target
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