MTHFS Protein (AA 1-203) (His tag)

Catalog No. ABIN2115468

Product Details

Target: MTHFS (5,10-Methenyltetrahydrofolate Synthetase (5-Formyltetrahydrofolate Cyclo-Ligase) (MTHFS))

Protein Type: Recombinant

Protein Characteristics: AA 1-203

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MTHFS protein is labelled with His tag.

Purpose: Recombinant Human 5-Formyltetrahydrofolate Cyclo-Ligase/MTHFS (C-6His)

Sequence: MAAAAVSSAK RSLRGELKQR LRAMSAEERL RQSRVLSQKV IAHSEYQKSK RISIFLSMQD EIETEEIIKD IFQRGKICFI PRYRFQSNHM DMVRIESPEE ISLLPKTSWN IPQPGEGDVR EEALSTGGLD LIFMPGLGFD KHGNRLGRGK GYYDAYLKRC LQHQEVKPYT LALAFKEQIC LQVPVNENDM KVDEVLYEDS STALEHHHHH H

Characteristics: Recombinant Human 5-formyltetrahydrofolate cyclo-ligase is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Ala203) of Human MTHFS fused with a 6His tag at the C-terminus..

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM Tris,200 mM Nacl,1 mM DTT,50 % Glycerol, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: MTHFS (5,10-Methenyltetrahydrofolate Synthetase (5-Formyltetrahydrofolate Cyclo-Ligase) (MTHFS))

Alternative Name: 5-Formyltetrahydrofolate Cyclo-Ligase (MTHFS Products)

Synonyms: MTHFS Protein, 1110034I12Rik Protein, 2310020H23Rik Protein, AI119695 Protein, HsT19268 Protein, 5,10-methenyltetrahydrofolate synthetase (5-formyltetrahydrofolate cyclo-ligase) Protein, methenyltetrahydrofolate synthetase Protein, 5-formyltetrahydrofolate cyclo-ligase (predicted) Protein, 5-formyltetrahydrofolate cyclo-ligase Protein, 5, 10-methenyltetrahydrofolate synthetase Protein, MTHFS Protein, SPBC1703.08c Protein, ZMO0215 Protein, LBA1507 Protein, Rxyl_1321 Protein, MEMAR_RS07640 Protein, Mjls_4669 Protein, STROP_RS03755 Protein, Mext_0779 Protein, AMF_RS07350 Protein, CKC_02040 Protein, Mthfs Protein

Background: 5-formyltetrahydrofolate cyclo-ligase (MTHFS) belongs to the 5-formyltetrahydrofolate cyclo-ligase family. It is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, contributes to tetrahydrofolate metabolism. MTHFS helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion.
Synonyms: 5-formyltetrahydrofolate cyclo-ligase,5,10-methenyl-tetrahydrofolate synthetase,MTHFS,Methenyl-THF synthetase

Molecular Weight: 24.3 kDa

UniProt: P49914