HMGB1 Protein
Quick Overview for HMGB1 Protein (ABIN2115470)
Target
See all HMGB1 ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Recombinant Human High Mobility Group Protein B1/HMGB1 (N-MARI)
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Sequence
- MARIDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAKG KPDAAKKGVV
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (GST tag)
HMGB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag,Biotin)HMGB1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
High Mobility Group Box 1 (HMGB1) (Non-oxidizable) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (Active) proteinHMGB1 Origin: Human, Mouse, Rat Host: Escherichia coli (E. coli) Recombinant > 95 % Act, CA Active
High Mobility Group Box 1 (HMGB1) (AA 1-90) protein (GST tag)HMGB1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
High Mobility Group Box 1 (HMGB1) (AA 1-215) protein (His tag)HMGB1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
High Mobility Group Box 1 (HMGB1) (Active) protein (Fc Tag)HMGB1 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
High Mobility Group Box 1 (HMGB1) (AA 1-215) proteinHMGB1 Origin: Human Host: Baculovirus Recombinant > 90 % pure SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of 50 mM HEPES-Na,p H 7.9,500 mM NaCl,0.6 mM DTT.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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Alternative Name
- High Mobility Group Protein B1
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Background
- Synonymes: High Mobility Group Protein B1, High Mobility Group Protein 1, HMG-1, HMGB1, HMG1
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Molecular Weight
- 10 kDa
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UniProt
- P09429
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Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
Target
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