IL12 Protein
Quick Overview for IL12 Protein (ABIN2129944)
Target
See all IL12 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Purification
- Purified by affinity chromatography.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- < 0.01 ng/μg cytokine as determined by the LAL assay.
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Interleukin 12 (IL12) (AA 23-328) (Active) protein (His-Avi Tag)
IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (Biotin,His-Avi Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Interleukin 12 (IL12) (AA 23-219), (AA 23-328) (Active) proteinIL12 Origin: Human Host: HEK-293T Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,DYKDDDDK Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)IL12 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 12 (IL12) (AA 23-219) protein (His tag,Fc Tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Interleukin 12 (IL12) proteinIL12 Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by SDS-PAGE
Interleukin 12 (IL12) (AA 23-328) (Active) protein (His tag)IL12 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS, AcA Active
Interleukin 12 (IL12) proteinIL12 Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Interleukin 12 (IL12) protein (His tag)IL12 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
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Application Notes
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WB: 1-10 μg/mL
It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest -
Comment
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Biological activity: The ED50 of this protein, as measured by IFN gamma induction assay in mouse splenocytes, is less than or equal to 175 pg/mL. This corresponds to a specific activity of greater than or equal to 5.7 x 10e6 Units/mg.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 500 μg/mL
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Buffer
- Supplied as a sterile liquid with 10 mM NaH2PO4, pH 6.0, 300 mM NaCl, with 1.0 % BSA. 0.22 μm filtered.
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Storage
- 4 °C/-20 °C/-80 °C
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- IL12 (Interleukin 12 (IL12))
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Alternative Name
- IL12
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Molecular Weight
- 65 kDa
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Pathways
- JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome
Target
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