Growth Hormone Receptor Protein (GHR) (AA 27-264) (His tag)

Catalog No. ABIN2181159

Product Details

Target: Growth Hormone Receptor (GHR)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 27-264

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Growth Hormone Receptor protein is labelled with His tag.

Sequence: AA 27-264

Characteristics: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.5 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Purity: >90 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Ko, Chandra, Ouyang, Kwon, Karande, Han: "Nanofluidic device for continuous multiparameter quality assurance of biologics." in: Nature nanotechnology, Vol. 12, Issue 8, pp. 804-812, (2019) (PubMed).

Nelson, Kim, Reuel, Salem, Bisker, Landry, Kruss, Barone, Kwak, Strano: "Mechanism of immobilized protein A binding to immunoglobulin G on nanosensor array surfaces." in: Analytical chemistry, Vol. 87, Issue 16, pp. 8186-93, (2015) (PubMed).

Target Details

Target: Growth Hormone Receptor (GHR)

Alternative Name: Growth Hormone R (GHR Products)

Background: Growth hormone receptor (GHR) is also known as somatotropin receptor, growth hormone-binding protein (GHBR), which belongs to the type I cytokine receptor family or Type 1 subfamily. GHR contains one fibronectin type-III domain. GHR / GHBR is expressed in various tissues with high expression in liver and skeletal muscle. The soluble form (GHBP) is produced by phorbol ester-promoted proteolytic cleavage at the cell surface (shedding) by ADAM17/TACE. GHR is receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.

Molecular Weight: 29.6 kDa

NCBI Accession: NP_000154

UniProt: P10912

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Response to Growth Hormone Stimulus