Growth Hormone Receptor Protein (GHR) (His tag)

Catalog No. ABIN7321234

This Recombinant Growth Hormone Receptor protein is expressed in HEK-293 Cells.

Quick Overview for Growth Hormone Receptor Protein (GHR) (His tag) (ABIN7321234)

Target: Growth Hormone Receptor (GHR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This Growth Hormone Receptor protein is labelled with His tag.

Purpose: Recombinant Rat Growth Hormone Receptor/GHR Protein (His Tag)(Active)

Sequence: Met 1-Arg 265

Characteristics: A DNA sequence encoding the rat GHR (P16310-1) extracellular domain (Met 1-Arg 265) was expressed, fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method

Biological Activity Comment: Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 0.5-2μg/mL in the presence of 50 ng/mL human growth hormone.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Growth Hormone Receptor (GHR)

Alternative Name: Growth Hormone Receptor/GHR

Background:

Background: Growth hormone receptor, also known as GH receptor and GHR, is a single-pass type I  membrane protein which belongs to the type I  cytokine receptor family and type 1 subfamily. GHR contains one fibronectin type-III domain. Growth hormone receptor / GHR is expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 of GHR is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression of GHR in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 of GHR is expressed in lung, stomach and muscle. Growth hormone receptor / GHR is a receptor for pituitary gland growth hormone. It is involved in regulating postnatal body growth. On ligand binding, it couples to the JAK2 / STAT5 pathway. Isoform 2 of GHR up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Defects in GHR are a cause of Laron syndrome (LARS) which is a severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may also be a cause of idiopathic short stature autosomal (ISSA) which is defined by a subnormal rate of growth.

Synonym: GHR/BP;MGC124963;MGC156665

Molecular Weight: 29.6 kDa

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Response to Growth Hormone Stimulus