Growth Hormone Receptor Protein (GHR) (His tag,Fc Tag)

Catalog No. ABIN7320419

This Recombinant Growth Hormone Receptor protein is expressed in HEK-293 Cells.

Quick Overview for Growth Hormone Receptor Protein (GHR) (His tag,Fc Tag) (ABIN7320419)

Target: Growth Hormone Receptor (GHR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 85 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This Growth Hormone Receptor protein is labelled with His tag,Fc Tag.

Purpose: Recombinant Mouse GHR/GHBP Protein (His & Fc Tag)(Active)

Sequence: Met 1-Gln 273

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Gln 273) of mouse GHR (NP_034414.2) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit proliferation of INS-1 cells induced by human growth hormone. The ED50 for this effect is 0.5-2 μg/mL in the presence of 50 ng/mL human growth hormone.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: Growth Hormone Receptor (GHR)

Alternative Name: GHR/GHBP

Background:

Background: Growth hormone receptor, also known as GH receptor and GHR, is a single-pass type I  membrane protein which belongs to the type I  cytokine receptor family and type 1 subfamily. GHR contains one fibronectin type-III domain. Growth hormone receptor / GHR is expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 of GHR is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression of GHR in placenta is predominant in chorion and decidua. Isoform 4 is highly expressed in placental villi. Isoform 2 of GHR is expressed in lung, stomach and muscle. Growth hormone receptor / GHR is a receptor for pituitary gland growth hormone. It is involved in regulating postnatal body growth. On ligand binding, it couples to the JAK2 / STAT5 pathway. Isoform 2 of GHR up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling. Defects in GHR are a cause of Laron syndrome (LARS) which is a severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may also be a cause of idiopathic short stature autosomal (ISSA) which is defined by a subnormal rate of growth.

Synonym: Growth Hormone Receptor;GHBP;GHR/BP

Molecular Weight: 56.8 kDa

NCBI Accession: NP_034414

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Response to Growth Hormone Stimulus