Growth Hormone Receptor Protein (GHR) (AA 27-264) (Fc Tag)

Catalog No. ABIN7092794

This Recombinant Growth Hormone Receptor protein is expressed in HEK-293 Cells.

Quick Overview for Growth Hormone Receptor Protein (GHR) (AA 27-264) (Fc Tag) (ABIN7092794)

Target: Growth Hormone Receptor (GHR)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 27-264

Purification tag / Conjugate: This Growth Hormone Receptor protein is labelled with Fc Tag.

Purpose: Recombinant Human GHR with C-terminal human Fc tag

Specificity: GHR(Ala27-Tyr264) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Growth Hormone Receptor (GHR)

Alternative Name: GHR

Background: GHBP,GHIP
This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

Molecular Weight: predicted molecular mass of 53.8 kDa after removal of the signal peptide. The apparent molecular mass of GHR-hFc is 55-100 kDa due to glycosylation.

UniProt: P10912

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Response to Growth Hormone Stimulus