Growth Hormone Receptor Protein (GHR) (AA 27-264) (His tag)

Catalog No. ABIN7274745

This Recombinant Growth Hormone Receptor protein is produced in HEK-293 Cells.

Quick Overview for Growth Hormone Receptor Protein (GHR) (AA 27-264) (His tag) (ABIN7274745)

Target: Growth Hormone Receptor (GHR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-264

Purification tag / Conjugate: This Growth Hormone Receptor protein is labelled with His tag.

Purpose: Human GHR/Growth Hormone R Protein

Sequence: Ala27-Tyr264

Characteristics: Recombinant Human GHR/Growth Hormone R Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Tyr264.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human GHR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human GHR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Growth Hormone Receptor (GHR)

Alternative Name: GHR

Background: Pegvisomant, a growth hormone receptor (GHR) antagonist, is a well-known drug that was designed to treat acromegaly. However, recent studies have indicated that the GHR is a "moonlighting" protein that may exhibit dual functions based on its localization in the plasma membrane and nucleus.

Molecular Weight: 28.8 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

UniProt: P10912

Pathways: NF-kappaB Signaling, JAK-STAT Signaling, Response to Growth Hormone Stimulus