IL-6 Protein (AA 30-212)

Catalog No. ABIN2181322

Recombinant IL-6 protein expressed in HEK-293 Cells and has been mentioned in 4+ publications.

Quick Overview for IL-6 Protein (AA 30-212) (ABIN2181322)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 30-212

Brand: ActiveMax®

Sequence: AA 30-212

Characteristics: This protein carries no "tag". The protein has a calculated MW of 20.8 kDa. The protein migrates as 24-27 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Gao, Lu, Guo, Xin: "Dual-optofluidic waveguide in-line fiber biosensor for real-time label-free detection of interferon-gamma with temperature compensation." in: Optics express, Vol. 28, Issue 7, pp. 10491-10504, (2021) (PubMed).

Lichtor, Chen, Elowe, Chen, Liu: "Side chain determinants of biopolymer function during selection and replication." in: Nature chemical biology, Vol. 15, Issue 4, pp. 419-426, (2019) (PubMed).

Hao, Pan, Huang, Wang, Zhao: "Sensitive detection of lung cancer biomarkers using an aptameric graphene-based nanosensor with enhanced stability." in: Biomedical microdevices, Vol. 21, Issue 3, pp. 65, (2019) (PubMed).

Hao, Pan, Shao, Lin, Zhao: "Graphene-based fully integrated portable nanosensing system for on-line detection of cytokine biomarkers in saliva." in: Biosensors & bioelectronics, Vol. 134, pp. 16-23, (2019) (PubMed).

Target Details

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL-6

Background: Interleukin 6 (IL-6) is also known as HGF, BSF2,HSF, IFNB2 and IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine that regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions.It is secreted by T cells, macrophages , monocytes, fibroblasts,endothelial cells,et.al. to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. Interleukin 6 has been shown to interact with interleukin-6 receptor and glycoprotein. IL-6 is relevant to many disease processes such as diabetes,atherosclerosis, depression,Alzheimer's Disease,systemic,lupus erythematosus,prostate cancer and rheumatoid arthritis. Advanced/metastatic cancer patients have higher levels of IL-6 in their blood.Hence there is an interest in developing anti-IL-6 agents as therapy against many of these diseases.

Molecular Weight: 20.8 kDa

NCBI Accession: NP_000591

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome