CD4 Protein (CD4) (AA 24-216) (His tag,Strep II tag)

Catalog No. ABIN2468318

Product Details

Target: CD4

Protein Type: Recombinant

Protein Characteristics: AA 24-216

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This CD4 protein is labelled with His tag,Strep II tag.

Application: Western Blotting (WB), ELISA, Mass Spectrometry (MS), Neutralization (Neut)

Purity: ~90 %

Application Details

Application Notes: This recombinant protein can be used for WB, ELISA, MS and neutralization assays.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM sodium phosphate, 500 mM NaCl, 25 mM imidazole, 10 % gycerol

Handling Advice: Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Storage: -80 °C

Storage Comment: Store in working aliquots at -70 °C.

Target Details

Target: CD4

Alternative Name: CD4 (CD4 Products)

Synonyms: cd4 Protein, L3T4 Protein, Ly-4 Protein, CD4mut Protein, W3/25 Protein, p55 Protein, CD4 molecule Protein, carcinoembryonic antigen-related cell adhesion molecule 5 Protein, T-cell surface glycoprotein CD4 Protein, CD4 antigen Protein, Cd4 molecule Protein, CD4 Protein, LOC100125525 Protein, cd4 Protein, Cd4 Protein

Background: CD4 is a member of the immunoglobulin superfamily and is implicated as associative recognition element in MHC (Major Histocompatibility Complex) class II-restricted immune response (1). On T-lymphocytes, it defines the helper/inducer subset. The mature 55 kd CD4 protein consists of a 372 amino acid extracellular segment composed of four tandem immunoglobulin-like VJ regions. The CD4 Molecule is a major receptor for human immunodeficiency virus (HIV), binding directly to the envelope glycoprotein gp120 on HIV, with the co-receptors being CCR5 or CXCR4. It has been shown that the V-like domains are critical for binding with HIV envelope gp120 (2). This soluble CD4 fragment (sCD4) will work in HIV neutralization assays at levels comparable to previously published results. The minor upper band seen in the Coomassie blue-stained gel is likely to be due to the presence of uncleaved signal sequence.

Molecular Weight: 27 kDa (Calculated)

Gene ID: 920

NCBI Accession: NP_000607

OMIM: 10835167

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events