Nanog Protein (TAT tag)
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- Target See all Nanog (NANOG) Proteins
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This Nanog protein is labelled with TAT tag.
- Sequence
- MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR
- Characteristics
- Endotoxin level is less than 0.1 ng per ug (1EU/μg).
- Purity
- < 95 % by SDS-PAGE gel and HPLC analyses.
- Endotoxin Level
- Endotoxin level is less than 0.1 ng per ug (1 EU/μg).
- Top Product
- Discover our top product NANOG Protein
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Nanog Homeobox (NANOG) (AA 2-305) protein (His tag)
NANOG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Nanog Homeobox (NANOG) (AA 1-305) proteinNANOG Origin: Human Host: Escherichia coli (E. coli) > 90 % by SDS - PAGE SDS
Nanog Homeobox (NANOG) (AA 98-195) protein (GST tag)NANOG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Nanog Homeobox (NANOG) (AA 1-305) protein (Strep Tag)Crystallography grade NANOG Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Nanog Homeobox (NANOG) (AA 1-305) protein (Strep Tag)Crystallography grade NANOG Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Handling Advice
- As with any protein, exposing Nanog-TAT recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
- Storage
- -20 °C
- Storage Comment
- The recombinant protein is stable for at least 2 years from date of receipt at -20 °C. Reconstituted Nanog-TAT is stable for at least 3 months when stored in working aliquots with a carrier protein at -20 °C.
- Expiry Date
- 24 months
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- Target
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- Alternative Name
- Nanog (NANOG Products)
- Synonyms
- 2410002E02Rik Protein, ENK Protein, ecat4 Protein, NANOG Protein, hacp Protein, wu:fd19e04 Protein, wu:fd20a07 Protein, zgc:193933 Protein, Nanog homeobox Protein, nanog homeobox Protein, Nanog Protein, NANOG Protein, nanog Protein
- Background
- Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.2 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide.
- Gene ID
- 388112
- OMIM
- 74762336
- UniProt
- Q6NSW7
- Pathways
- Stem Cell Maintenance
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