TNF alpha Protein (AA 77-233)

Catalog No. ABIN2666981

This Recombinant TNF alpha protein is expressed in Escherichia coli (E. coli).

Quick Overview for TNF alpha Protein (AA 77-233) (ABIN2666981)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Biochemical Assay (BCA)

Purity: > 98 % , as determined by SDS-PAGE, Coomassie stained with Invitrogen SimplyBlue SafeStain.

Product Details

Protein Characteristics: AA 77-233

Endotoxin Level:

Less than 0.01ng per μg cytokine as determined by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.

Buffer: Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA.

Handling Advice: Use a manual defrost freezer and avoid repeated freeze thaw cycles.

Storage: 4 °C

Storage Comment: Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF-alpha

Background: TNF-α is released from macrophages, monocytes, neutrophils, T-cells (principally CD4+), NK-cells and many transformed cell lines. Soluble homotrimeric TNF-α is released from cells by proteolysis of the integral membrane precursor form of TNF-α. TNF-α binding to some TNF-α receptors induces apoptosis and depending on cell type, receptor expression, and signal transduction status can induce other responses. TNF-α is involved in the inflammatory response.

Molecular Weight: The 157 amino acid recombinant protein has a predicted molecular mass of 17,352 Da. The DTT-reduced and non-reduced protein migrates at approximately 16kDa by SDS-PAGE. The N-terminal amino acid is Val.

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome