ICOS Protein (AA 21-141, C-Term) (Fc Tag)

Catalog No. ABIN2667431

This Recombinant ICOS protein is expressed in Escherichia coli (E. coli).

Quick Overview for ICOS Protein (AA 21-141, C-Term) (Fc Tag) (ABIN2667431)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >95 % , as determined by Coomassie stained SDS-PAGE.

Product Details

Protein Characteristics: AA 21-141, C-Term

Purification tag / Conjugate: This ICOS protein is labelled with Fc Tag.

Sterility: 0.22 μm filtered

Endotoxin Level:

Less than 0.1 EU per μg of protein as determine by the LAL method.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: Immobilized human ICOS-Fc Chimera at 1 μg/mL (100 μL/well) can bind recombinant human B7-H2-Fc in a dose dependent manner with a kD < 5 nM.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: For maximum results, quick spin vial prior to opening. Stock solutions should be prepared at no less than 10 μg/mL in sterile buffer (PBS, HPBS, DPBS, and EBSS) containing carrier protein such as 1 % BSA or HSA. After dilution, the cytokine can be stored between 2 °C and 8 °C for one month or from -20 °C to -70 °C for up to 3 months.

Concentration: 200 μg/mL

Buffer: 0.22 μm filtered protein solution is in PBS

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored between 2°C and 8°C for three months, at -20°C for six months, or at -70°C for one year.

Target Details

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS

Background: Inducible costimulatory molecule (ICOS) is a member of CD28 superfamily protein. It has unique roles in T cell activation and differentiation, splenic germinal center formation, and immunoglobulin class switching. The expression of ICOS within the first 48 hours after T cell activation suggests that it may contribute to the differentiation of T cells during the primary response. ICOS binds to its ligand B7H, which is constitutively expressed at low levels on the surface of APCs and is upregulated by TNF-α or lipopolysaccharide. It was demonstrated that ICOS-B7H2 interaction plays an important role in the generation of CD4+ and CD8+ Tregs, and mediates the suppressive functions of CD4+ Tregs by regulating CTLA-4 expression on cell surfaces induced by allogeneic CD40-activated B cells. In the ICOS-KO mice, the T cell-mediated isotype switching is impaired while the IFN-gamma response is slightly affected, in addition, the ICOS-negative T cells do not accumulate in B cell follicles. Also, secondary, but not primary, clonal proliferation of antigen-specific B cells is impaired in ICOS-KO mice, and therefore the generation of secondary antibody-secreting cells is also impaired. These results suggest that this protein plays a pivotal role in T cell activation and Th1/Th2 differentiation.

Molecular Weight: The 359 amino acid recombinant protein with a C-terminal human IgG1 Fc tag has a predicted molecular mass of approximately 44 kDa. The protein migrates above 60 kDa in SDS-PAGE in DTT-reducing condition and above 120 kDa in non-reducing condition. The N-t

Pathways: Cancer Immune Checkpoints