IL-11 Protein (AA 22-199)

Catalog No. ABIN2667453

Recombinant IL-11 protein expressed in Escherichia coli (E. coli).

Quick Overview for IL-11 Protein (AA 22-199) (ABIN2667453)

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Flow Cytometry (FACS)

Purity: >98 % , as determined by Coomassie stained SDS-PAGE and HPLC analysis.

Product Details

Protein Characteristics: AA 22-199

Endotoxin Level:

Less than 0.1 ng per μg of protein.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Biological activity: ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5.0 x 105 units/mg as determined by the dose-dependent stimulation of the proliferation of murine T11 cells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For maximum results, quick spin vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer, such as 5 % Trehalose, and store working aliquots at -20 °C to -80 °C.

Buffer: Lyophilized, carrier-free.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Unopened vial can be stored at -20°C or -70°C.

Target Details

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Alternative Name: IL-11

Background: IL-11, a member of the IL-6 family of cytokines, exerts a wide range of biological effects on various cell types including hematopoietic cells, hepatocytes, adipocytes, neurons, and osteoblasts. IL-11 works synergistically with other growth factors including SCF, IL-4, IL-3, IL-7, IL-12, IL-13, and GM-CSF to stimulate the proliferation of cells from several hematopoietic lineages. Binding of IL-11 to IL-11 receptor (IL-11R) induces membrane bound gp130 homodimerization and triggers STAT3 phosphorylation by JAK. IL-11 shares the common receptor subunit gp130 with IL-6, IL-27, LIF, OSM, CNTF, CT-1, CLC, and NP. Female mice deficient in IL-11R revealed an important role for IL-11 in embryonic implantation. In addition, IL-11 shows anti-inflammatory activity in models of inflammatory bowel disease, chemotherapy induced oral mucositis, and inflammatory arthritis. IL-11 and IL-13 are highly expressed in asthmatic airways (Th2 response). IL-11 can inhibit Th1 responses and the production of Th1 cytokines such as IL-12, shifting inflammation in Th2 direction. Elevated IL-11 expression is associated with tumor grade and invasion in gastric cancer. Recombinant human IL-11 has been clinically approved to improve platelet recovery after chemotherapy-induced thrombocytopenia.

Molecular Weight: The 179 amino acid N-terminal methionylated recombinant protein has a prodicted molecular mass of 19.1 kDa. The predicted N-terminal amino acid is Met.

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion