IL-11 Protein (AA 22-199) (His tag)

Catalog No. ABIN6239760

Product Details

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Biological Activity: Active

Protein Characteristics: AA 22-199

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This IL-11 protein is labelled with His tag.

Application: Activity Assay (AcA), Cell Culture (CC)

Characteristics: Tag location: N-terminal His Tag

Purity: > 90 %

Biological Activity Comment: IL11 (Interleukin-11) is a multifunctional cytokine first isolated from bone marrow-derived stromal cells. It stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Besides, IL11 is reported to induce the proliferation of human T-cells, thus, a proliferation assay was conducted to detect the bioactivity of human recombinant IL11 using Jurkat cells. Briefly, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 10, 000 cells/well in RPMI-1640 with the addition of various concentrations of IL11. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Cell proliferation of Jurkat cells after incubation with IL11 for 72h observed by inverted microscope was shown in Figure 1. The CCK-8 data was shown in Figure 2. It was obvious that IL11 significantly promoted cell proliferation of Jurkat cells.

Application Details

Application Notes: Isoelectric Point: 11.3

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Target Details

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Abstract: IL11 Products

Synonyms: IL11 Protein, AGIF Protein, IL-11 Protein, Il-11 Protein, interleukin 11 Protein, IL11 Protein, Il11 Protein

Background: Alternative Names: AGIF, Adipogenesis Inhibitory Factor, Oprelvekin

Molecular Weight: 27kDa

UniProt: P20809

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion