IL-11 Protein (AA 22-199) (His tag)

Catalog No. ABIN7274885

Recombinant IL-11 protein expressed in HEK-293 Cells.

Quick Overview for IL-11 Protein (AA 22-199) (His tag) (ABIN7274885)

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-199

Purification tag / Conjugate: This IL-11 protein is labelled with His tag.

Purpose: Human IL-11 Protein

Sequence: Pro22-Leu199

Characteristics: Recombinant Human IL-11 Protein is expressed from HEK293 with His tag at the N-Terminus, It contains Pro22-Leu199.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind Human IL-11, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-11, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 8.1 ng/ml determined by ELISA (QC Test).,Human IL-11 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured in a cell proliferation assay using TF-1 cells. The ED50-for this effect is typically 1-8 ng/mL.,The purity of Human IL-11 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL-11 (IL11) (Interleukin 11 (IL11))

Alternative Name: IL-11

Background: Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers, human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.

Molecular Weight: 20.24 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Bis-Tris PAGE result.

UniProt: P20809

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion