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FAK Protein (AA 2-1052, GST-His-Tag) (GST-His-Tag)

PTK2 Origin: Human Host: Baculovirus Recombinant WB, KAA Active
Catalog No. ABIN2669460
  • Target See all FAK (PTK2) Proteins
    FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 2-1052, GST-His-Tag
    Origin
    • 13
    • 2
    • 2
    Human
    Source
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    Baculovirus
    Purification tag / Conjugate
    This FAK protein is labelled with GST-His-Tag.
    Application
    Western Blotting (WB), Kinase Activity Assay (KAA)
    Characteristics
    Recombinant human FAK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids A2-H1052, accession number L13616 and N-terminally fused to GST-HIS6TagTagTag-Thrombin cleavage site. Purified by GSH-agarose affinity purification.
    Purification
    Purified by GSH-agarose affinity purification.
    Top Product
    Discover our top product PTK2 Protein
  • Application Notes
    Recombinant FAK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~145.394 kDa. The activity of the protein is ~ 1.5 pmol/μg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 μM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 μg/50 μL PEG20.000, Substrate: PolyEY 0.5 μg/50 μL, Recombinant FAK: 200 ng/50 μL. Kinase activity may vary depending on the substrate and reaction conditions used.
    Restrictions
    For Research Use only
  • Concentration
    0.135 μg/μL
  • Target
    FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))
    Alternative Name
    FAK (PTK2 Products)
    Synonyms
    FADK Protein, FAK Protein, FAK1 Protein, FRNK Protein, PPP1R71 Protein, p125FAK Protein, pp125FAK Protein, PTK2 Protein, Fadk Protein, mKIAA4203 Protein, fak Protein, fak1a Protein, ptk2 Protein, ptk2a Protein, wu:fa18c04 Protein, zgc:66114 Protein, CG10023 Protein, CT28129 Protein, DFAK Protein, DFak Protein, DFak56 Protein, Dfak Protein, Dfak56 Protein, DmFAK Protein, Dmel\\CG10023 Protein, Fak56 Protein, Fak56D Protein, fak56 Protein, protein tyrosine kinase 2 Protein, protein tyrosine kinase 2 L homeolog Protein, PTK2 protein tyrosine kinase 2 Protein, protein tyrosine kinase 2ab Protein, Focal adhesion kinase Protein, PTK2 Protein, ptk2.L Protein, Ptk2 Protein, ptk2ab Protein, Fak Protein
    Pathways
    Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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