PRC2 Complex (full length ) protein (DYKDDDDK Tag)
Origin: Human
Host: Baculovirus
Recombinant
EAA, ScA
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- Target
- PRC2 Complex
- Protein Type
- Recombinant
- Protein Characteristics
- full length
- Origin
- Human
- Source
- Baculovirus
- Purification tag / Conjugate
- DYKDDDDK Tag
- Application
- Enzyme Activity Assay (EAA), Screening Assay (ScA)
- Characteristics
- Recombinant PRC2 Complex that includes full length EZH2, SUZ12, EED and RbAp46/48 (accession numbers NP_001190176.1, NP_056170, NP_003788.2, NP_002884.1, and NP_005601.1, respectively) was expressed in Sf9 and contains an N-terminal FLAG tag at the N-terminus of EZH2. The molecular weights of expressed EZH2, SUZ12, EED and RbAp46/48 are 87 kDa, 83 kDa, 50.2 kDa, 47.8 kDa and 47.7 kDa, respectively.
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- Application Notes
- PRC2 Complex is suitable for use in the study of enzyme kinetics, inhibitor screening and selectivity profiling. Specific Activity: H3K27me(1-3) methyltransferase. HMT Assay Conditions: 50 mM TrisCl, pH 8.6, 0.02 % Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM, 3.3 μM H3K27me0 peptide, 40 nM Recombinant PRC2 Complex at 2 hours at room temperature. Activity was detected by MALDI-TOF.
- Restrictions
- For Research Use only
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- Handling Advice
- Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
- Storage
- -80 °C
- Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation.
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- Target
- PRC2 Complex
- Background
- PRC2 (Polycomb Repressive Complex 2) is one of the two classes Polycomb-group, or PcG proteins (the other being PRC1) that are important epigenetic determinants of stem cell identity. They play an important role in long-term epigenetic silencing of genes during cell fate determination and differentiation. PRC2 functions as a repressor of chromatin. PRC2 is required to target recruitment to specific DNA sequences (termed Polycomb Response Elements or PREs) of genomic regions to be silenced. Once associated with chromatin, the PRC2 subunit EZH2 has histone methyltransferase activity that catalyzes the trimethylation of histone H3 at lysine 27. H3K27me3 is well established as a hallmark of regions of repressed chromatin. Trimethylation of lysine 27 leads to the recruitment of PRC1 through the binding of H3K27me3 by chromodomain-containing proteins in PRC1. PRC1 is responsible for long-term gene silencing after cellular differentiation.
- Molecular Weight
- The molecular weights of expressed EZH2, SUZ12, EED and RbAp46/48 are 87 kDa, 83 kDa, 50.2 kDa, 47.8 kDa and 47.7 kDa, respectively.
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