LOX Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2712316

This Recombinant LOX protein is expressed in HEK-293 Cells and has been mentioned in 12+ publications.

Quick Overview for LOX Protein (Myc-DYKDDDDK Tag) (ABIN2712316)

Target: LOX (Lysyl Oxidase (LOX))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This LOX protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Lysyl oxidase protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Pilecki, Holm, Schlosser, Moeller, Wohl, Zuk, Heumüller, Wallis, Moestrup, Sengle, Holmskov, Sorensen: "Characterization of Microfibrillar-associated Protein 4 (MFAP4) as a Tropoelastin- and Fibrillin-binding Protein Involved in Elastic Fiber Formation." in: The Journal of biological chemistry, Vol. 291, Issue 3, pp. 1103-14, (2016) (PubMed).

Kondala, Puri, Banka, Sachdeva, Sakhuja: "Short-term prognosis of potential celiac disease in Indian patients." in: United European gastroenterology journal, Vol. 4, Issue 2, pp. 275-80, (2016) (PubMed).

Cox, Rumney, Schoof, Perryman, Høye, Agrawal, Bird, Latif, Forrest, Evans, Huggins, Lang, Linding, Gartland, Erler: "The hypoxic cancer secretome induces pre-metastatic bone lesions through lysyl oxidase." in: Nature, Vol. 522, Issue 7554, pp. 106-10, (2015) (PubMed).

Miller, Morton, Pinese, Saturno, Jamieson, McGhee, Timpson, Leach, McGarry, Shanks, Bailey, Chang, Oien, Karim, Au, Steele, Carter, McKay, Anderson, Evans, Marais, Springer, Biankin, Erler, Sansom: "Targeting the LOX/hypoxia axis reverses many of the features that make pancreatic cancer deadly: inhibition of LOX abrogates metastasis and enhances drug efficacy." in: EMBO molecular medicine, Vol. 7, Issue 8, pp. 1063-76, (2015) (PubMed).

Richter, Dayaram, Gilmartin, Ganji, Pemmasani, Van Der Key, Shohet, Donehower, Kumar: "WIP1 phosphatase as a potential therapeutic target in neuroblastoma." in: PLoS ONE, Vol. 10, Issue 2, pp. e0115635, (2015) (PubMed).

Zhao, Sagare, Ma, Halliday, Kong, Kisler, Winkler, Ramanathan, Kanekiyo, Bu, Owens, Rege, Si, Ahuja, Zhu, Miller, Schneider, Maeda, Maeda, Sugawara, Ichida, Zlokovic: "Central role for PICALM in amyloid-β blood-brain barrier transcytosis and clearance." in: Nature neuroscience, Vol. 18, Issue 7, pp. 978-87, (2015) (PubMed).

Arnold, Kent, Hogarth, Schlatt, Prasad, Haenisch, Walsh, Muller, Griswold, Amory, Isoherranen: "Importance of ALDH1A enzymes in determining human testicular retinoic acid concentrations." in: Journal of lipid research, Vol. 56, Issue 2, pp. 342-57, (2015) (PubMed).

Wilson, Bradley, Shaw, Wagenmakers: "Paxillin and focal adhesion kinase colocalise in human skeletal muscle and its associated microvasculature." in: Histochemistry and cell biology, Vol. 142, Issue 3, pp. 245-56, (2014) (PubMed).

Nandhu, Hu, Cole, Erdreich-Epstein, Rodriguez-Gil, Viapiano: "Novel paracrine modulation of Notch-DLL4 signaling by fibulin-3 promotes angiogenesis in high-grade gliomas." in: Cancer research, Vol. 74, Issue 19, pp. 5435-48, (2014) (PubMed).

Divakaran, Hines, McCarver: "Human hepatic UGT2B15 developmental expression." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 141, Issue 1, pp. 292-9, (2014) (PubMed).

Tansley, Betteridge, Gunawardena, Jacques, Owens, Pilkington, Arnold, Yasin, Moraitis, Wedderburn, McHugh: "Anti-MDA5 autoantibodies in juvenile dermatomyositis identify a distinct clinical phenotype: a prospective cohort study." in: Arthritis research & therapy, Vol. 16, Issue 4, pp. R138, (2014) (PubMed).

Baker, Bird, Welti, Gourlaouen, Lang, Murray, Reynolds, Cox, Erler: "Lysyl oxidase plays a critical role in endothelial cell stimulation to drive tumor angiogenesis." in: Cancer research, Vol. 73, Issue 2, pp. 583-94, (2013) (PubMed).

Target Details

Target: LOX (Lysyl Oxidase (LOX))

Alternative Name: Lysyl Oxidase

Background: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. In addition to cross-linking of extracellular matrix proteins, may have a direct role in tumor suppression. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 46.8 kDa

NCBI Accession: NP_002308

Pathways: SARS-CoV-2 Protein Interactome