MRPL47 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all MRPL47 products
- MRPL47 (Mitochondrial Ribosomal Protein L47 (MRPL47))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
- Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MRPL47 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human MRPL47 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- MRPL47 (Mitochondrial Ribosomal Protein L47 (MRPL47))
- Alternative Name
- Mrpl47 (MRPL47 Products)
- Synonyms
- BcDNA:AT29239 Protein, CG9378 Protein, Dmel\\CG9378 Protein, MRP-L47 Protein, Rlc1 Protein, L47mt Protein, NCM1 Protein, 4833424P18Rik Protein, CGI-204 Protein, ENSMUSG00000051761 Protein, Gm9859 Protein, MTF/L47 Protein, MRPL47 Protein, id:ibd1090 Protein, mitochondrial ribosomal protein L47 Protein, 39S ribosomal protein L47, mitochondrial Protein, mRpL47 Protein, MRPL47 Protein, Mrpl47 Protein, LOC413774 Protein, mrpl47 Protein
- Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified.
- Molecular Weight
- 16.8 kDa
- NCBI Accession
- NP_817125
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