RASGRF1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
This Recombinant RASGRF1 protein is produced in HEK-293 Cells.
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Catalog No. ABIN2712357
$956.40
Plus shipping costs $50.00, if applicable $20.00 dry ice
20 μg
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United States
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Quick Overview for RASGRF1 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2712357)
Target
See all RASGRF1 Proteins
RASGRF1
(Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))
Protein Type
Recombinant
Origin
Human
Source
HEK-293 Cells
Application
Antibody Production (AbP), Standard (STD)
Purity
> 80 % as determined by SDS-PAGE and Coomassie blue staining
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This RASGRF1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human RASGRF1 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 1-110) protein (GST tag)
RASGRF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))
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Alternative Name
- Rasgrf1
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Background
- The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
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Molecular Weight
- 145.1 kDa
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NCBI Accession
- NP_002882
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Pathways
- RTK Signaling, Cell Division Cycle, M Phase
Target
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