PYCARD Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
Quick Overview for PYCARD Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2713165)
Target
See all PYCARD ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This PYCARD protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human CARD5 / PYCARD / ASC (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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PYD and CARD Domain Containing (PYCARD) (AA 1-195) protein (His tag)
custom-made PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PYD and CARD Domain Containing (PYCARD) (AA 1-193) protein (His tag)custom-made PYCARD Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
PYD and CARD Domain Containing (PYCARD) (AA 1-149) protein (GST tag)PYCARD Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- PYCARD (PYD and CARD Domain Containing (PYCARD))
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Alternative Name
- Card5,pycard,asc
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Background
- This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 21.4 kDa
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NCBI Accession
- NP_037390
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Pathways
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
Target
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