KIT Protein (DYKDDDDK Tag)
Quick Overview for KIT Protein (DYKDDDDK Tag) (ABIN2713384)
Target
See all KIT ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This KIT protein is labelled with DYKDDDDK Tag.
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Characteristics
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- Recombinant human CD117 / c-kit (C-term DDK tag, transcript variant 1) protein expressed in sf9 cells.
- Produced with end-sequenced ORF clone
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Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-520) protein (His tag)
KIT Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-516) (Active) protein (Fc Tag)KIT Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 1-976) protein (GST tag)KIT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Mast/stem Cell Growth Factor Receptor (KIT) protein (His tag)KIT Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Mast/stem Cell Growth Factor Receptor (KIT) (Active) protein (His tag)KIT Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Mast/stem Cell Growth Factor Receptor (KIT) protein (Fc Tag)KIT Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Mast/stem Cell Growth Factor Receptor (KIT) (Active) protein (Fc Tag)KIT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-524) protein (His tag)KIT Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Mast/stem Cell Growth Factor Receptor (KIT) (AA 26-516) (Active) protein (His tag)KIT Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- KIT (Mast/stem Cell Growth Factor Receptor (KIT))
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Alternative Name
- Cd117,c-Kit
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Background
- This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 48.7 kDa
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NCBI Accession
- NP_000213
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
Target
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