Thrombomodulin Protein (THBD)
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- Target See all Thrombomodulin (THBD) Proteins
- Thrombomodulin (THBD)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Application
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human CD141 / THBD protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Measured by its ability to activate protein C induced cleavage of the chromogenic substrate, BOC-Asp-Pro Arg-AMC in the presence of thrombin. The specific activity is greater than 500 pmoles/min/ug.
- Top Product
- Discover our top product THBD Protein
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Thrombomodulin (THBD) protein (His tag)
THBD Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Thrombomodulin (THBD) protein (His tag)THBD Origin: Rat Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Thrombomodulin (THBD) (AA 19-515) protein (His tag)Crystallography grade THBD Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Thrombomodulin (THBD) (AA 17-517) protein (His tag)Crystallography grade THBD Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Thrombomodulin (THBD) (AA 19-575) protein (rho-1D4 tag)Crystallography grade THBD Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Thrombomodulin (THBD) (AA 17-577) protein (rho-1D4 tag)Crystallography grade THBD Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Thrombomodulin (THBD)
- Alternative Name
- Cd141,thbd (THBD Products)
- Synonyms
- AHUS6 Protein, BDCA3 Protein, CD141 Protein, THPH12 Protein, THRM Protein, TM Protein, AI385582 Protein, THBD Protein, HAST Protein, HAST3 Protein, M-PST Protein, ST1A3/ST1A4 Protein, ST1A5 Protein, STM Protein, TL-PST Protein, thrombomodulin Protein, sulfotransferase family 1A member 3 Protein, THBD Protein, Thbd Protein, SULT1A3 Protein
- Background
- Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. [UniProtKB/Swiss-Prot Function]
- Molecular Weight
- 51 kDa
- NCBI Accession
- NP_000352
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