ACTN1 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
-
- Target See all ACTN1 Proteins
- ACTN1 (Actinin, alpha 1 (ACTN1))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ACTN1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human Alpha-actinin-1 / ACTN1 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ACTN1 Protein
-
Actinin, alpha 1 (ACTN1) (AA 272-490) protein (His-IF2DI Tag)
ACTN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Actinin, alpha 1 (ACTN1) (AA 1-892) protein (GST tag)ACTN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Actinin, alpha 1 (ACTN1) (AA 543-639) protein (GST tag)ACTN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Actinin, alpha 1 (ACTN1) (AA 1-892) protein (Strep Tag)Crystallography grade ACTN1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Actinin, alpha 1 (ACTN1) (AA 1-892) protein (Strep Tag)Crystallography grade ACTN1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- ACTN1 (Actinin, alpha 1 (ACTN1))
- Alternative Name
- alpha-Actinin-1,actn1 (ACTN1 Products)
- Synonyms
- ACTN1 Protein, actinin Protein, BDPLT15 Protein, 3110023F10Rik Protein, Actn1a Protein, actinin alpha 1 Protein, actinin, alpha 1 Protein, actinin alpha 1 L homeolog Protein, ACTN1 Protein, actn1 Protein, Actn1 Protein, actn1.L Protein
- Background
- Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 102.9 kDa
- NCBI Accession
- NP_001093
- Pathways
- Cell-Cell Junction Organization
-
