ATP Synthase Subunit beta (AtpB) protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2715031

This Recombinant protein is expressed in HEK-293 Cells.

Quick Overview for ATP Synthase Subunit beta (AtpB) protein (Myc-DYKDDDDK Tag) (ABIN2715031)

Target: ATP Synthase Subunit beta (AtpB)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: Myc-DYKDDDDK Tag

Characteristics:

• Recombinant human ATP synthase subunit beta protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: ATP Synthase Subunit beta (AtpB)

Alternative Name: Atp Synthase Subunit beta

Background: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 51.7 kDa

NCBI Accession: NP_001677

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process