ATP5E Protein (Myc-DYKDDDDK Tag)
-
- Target See all ATP5E Proteins
- ATP5E (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This ATP5E protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human ATP synthase subunit epsilon protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product ATP5E Protein
-
ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E) (AA 1-51) protein (GST tag)
ATP5E Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E) (AA 2-51) protein (His tag)Crystallography grade ATP5E Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E) (AA 2-52) protein (His tag)Crystallography grade ATP5E Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the C-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- ATP5E (ATP Synthase, H+ Transporting, Mitochondrial F1 Complex, epsilon Subunit (ATP5E))
- Alternative Name
- Atp Synthase Subunit epsilon (ATP5E Products)
- Synonyms
- 2410043G19Rik Protein, ATPE Protein, AV000645 Protein, MC5DN3 Protein, DDBDRAFT_0217763 Protein, DDBDRAFT_0233319 Protein, DDB_0217763 Protein, DDB_0233319 Protein, ATP5E Protein, ATPsynepsilon Protein, BcDNA:RE19513 Protein, BcDNA:RH48911 Protein, CG9032 Protein, Dmel\CG9032 Protein, Sun Protein, l(1)13Fe Protein, l(1)XV Protein, ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit Protein, ATP synthase epsilon chain, mitochondrial Protein, mitochondrial F1F0 ATP synthase epsilon subunit Protein, ATP synthase epsilon chain Protein, stunted Protein, Atp5e Protein, ATP5E Protein, atp5e Protein, LOC100347099 Protein, meatpase Protein, atpE Protein, sun Protein
- Background
- This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. Read-through transcripts that include exons from this gene are expressed from the upstream gene SLMO2.[provided by RefSeq, Mar 2011].
- Molecular Weight
- 5.6 kDa
- NCBI Accession
- NP_008817
- Pathways
- Proton Transport, Ribonucleoside Biosynthetic Process
-
