ERCC8 Protein (His tag)
Quick Overview for ERCC8 Protein (His tag) (ABIN2720444)
Target
See all ERCC8 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This ERCC8 protein is labelled with His tag.
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Characteristics
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- Recombinant human ERCC8 (full length, N-term HIS tag, transcript variant 3) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8) (AA 1-397) protein (His tag)
custom-made ERCC8 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8) (AA 1-396) protein (GST tag)ERCC8 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8) (AA 1-205) protein (GST tag)ERCC8 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the N-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))
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Alternative Name
- Ercc8
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Background
- This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 23.2 kDa
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NCBI Accession
- NP_001007235
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Pathways
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
Target
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