FGF9 Protein

Catalog No. ABIN2721101

Product Details

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)

Specificity: Optimal preservation of protein structure, post-translational modifications and functions.

Characteristics:

• Recombinant human FGF9 protein expressed in E. coli.
• Produced with end-sequenced ORF clone
• Tested for bioactivity.

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Endotoxin Level: Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Biological Activity Comment: ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is less than or equal to 0.5 ng/ml, corresponding to a specific activity of > 2 X 10^6 units/mg.

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays

Restrictions: For Research Use only

Handling

Buffer: Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2

Handling Advice: Resuspend the protein in the desired concentration in proper buffer

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: FGF9 (FGF-9) (Fibroblast Growth Factor 9 (FGF-9))

Alternative Name: Fgf9 (FGF-9 Products)

Synonyms: Eks Protein, FGF-9 Protein, GAF Protein, HBFG-9 Protein, HBGF-9 Protein, SYNS3 Protein, XFGF-9 Protein, fgf9-A Protein, fibroblast growth factor 9 Protein, fibroblast growth factor 9 S homeolog Protein, Fgf9 Protein, FGF9 Protein, fgf9.S Protein

Background: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.

Molecular Weight: 23.2 kDa

NCBI Accession: NP_002001