GAD65 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)
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- Target See all GAD65 (GAD2) Proteins
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- Protein Type
- Recombinant
- Protein Characteristics
- Transcript Variant 2
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This GAD65 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human GAD2 / GAD65 (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product GAD2 Protein
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Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) protein (GST tag)
GAD2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) proteinGAD2 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) protein (GST tag,His tag)GAD2 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 92 % as determined by SDS-PAGE
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) protein (GST tag)GAD2 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) protein (Strep Tag)Crystallography grade GAD2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) protein (Strep Tag)Crystallography grade GAD2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585), (full length) protein (His tag)GAD2 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- Alternative Name
- Gad2,gad65 (GAD2 Products)
- Synonyms
- GAD65 Protein, gad65 Protein, F12P19.12 Protein, F12P19_12 Protein, GLUTAMATE DECARBOXYLASE 2 Protein, glutamate decarboxylase 2 Protein, zgc:112198 Protein, 6330404F12Rik Protein, GAD(65) Protein, Gad-2 Protein, glutamate decarboxylase 2 Protein, glutamate decarboxylase 1 Protein, glutamic acid decarboxylase 2 Protein, GAD2 Protein, PTRG_06148 Protein, LOC9324534 Protein, Gad2 Protein, GAD1 Protein, gad2 Protein
- Background
- This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
- Molecular Weight
- 65.2 kDa
- NCBI Accession
- NP_001127838
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