Osteoactivin Protein (GPNMB) (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for Osteoactivin Protein (GPNMB) (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2722179)
Target
See all Osteoactivin (GPNMB) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This Osteoactivin protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human GPNMB / HGFIN (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Osteoactivin (GPNMB) (AA 23-502) (Active) protein (His tag)
GPNMB Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Osteoactivin (GPNMB) (AA 23-486) (Active) protein (His tag)GPNMB Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Osteoactivin (GPNMB) (AA 23-496) protein (His tag)GPNMB Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Osteoactivin (GPNMB) (AA 1-572) protein (GST tag)GPNMB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Osteoactivin (GPNMB) (AA 22-474) protein (His tag)GPNMB Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Osteoactivin (GPNMB) (AA 22-474) protein (His tag)GPNMB Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Osteoactivin (GPNMB) protein (His tag)GPNMB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer." in: Nature cell biology, Vol. 18, Issue 2, pp. 213-24, (2016) (PubMed).
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: "The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer." in: Nature cell biology, Vol. 18, Issue 2, pp. 213-24, (2016) (PubMed).
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- Osteoactivin (GPNMB)
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Alternative Name
- Gpnmb,hgfin
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Background
- The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 61.5 kDa
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NCBI Accession
- NP_001005340
Target
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