Osteoactivin Protein (GPNMB) (AA 22-474) (His tag)
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- Target See all Osteoactivin (GPNMB) Proteins
- Osteoactivin (GPNMB)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-474
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Osteoactivin protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Sequence
- ADPAKRFHDV LGNERPSAYM REHNQLNGWS SDENDWNEKL YPVWKRGDMR WKNSWKGGRV QAVLTSDSPA LVGSNITFAV NLIFPRCQKE DANGNIVYEK NCRNEAGLSA DPYVYNWTAW SEDSDGENGT GQSHHNVFPD GKPFPHHPGW RRWNFIYVFH TLGQYFQKLG RCSVRVSVNT ANVTLGPQLM EVTVYRRHGR AYVPIAQVKD VYVVTDQIPV FVTMFQKNDR NSSDETFLKD LPIMFDVLIH DPSHFLNYST INYKWSFGDN TGLFVSTNHT VNHTYVLNGT FSLNLTVKAA APGPCPPPPP PPRPSKPTPS LGPAGDNPLE LSRIPDENCQ INRYGHFQAT ITIVEGILEV NIIQMTDVLM PVPWPESSLI DFVVTCQGSI PTEVCTIISD PTCEITQNTV CSPVDVDEMC LLTVRRTFNG SGTYCVNLTL GDDTSLALTS TLISVPHHHH HH
- Purity
- > 90 % by SDS - PAGE
- Endotoxin Level
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product GPNMB Protein
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Osteoactivin (GPNMB) (AA 23-502) protein (His tag)
GPNMB Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Osteoactivin (GPNMB) (AA 23-486) protein (His tag)GPNMB Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Osteoactivin (GPNMB) (AA 1-572) protein (GST tag)GPNMB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Osteoactivin (GPNMB) (AA 104-203) protein (GST tag)GPNMB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Osteoactivin (GPNMB) (AA 22-486) protein (His tag)Crystallography grade GPNMB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Osteoactivin (GPNMB) (AA 23-502) protein (His tag)Crystallography grade GPNMB Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Osteoactivin (GPNMB) (AA 22-572) protein (rho-1D4 tag)Crystallography grade GPNMB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Osteoactivin (GPNMB) (AA 23-574) protein (rho-1D4 tag)Crystallography grade GPNMB Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Osteoactivin (GPNMB) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GPNMB Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- Target
- Osteoactivin (GPNMB)
- Alternative Name
- GPNMB (GPNMB Products)
- Synonyms
- HGFIN Protein, NMB Protein, DC-HIL Protein, Dchil Protein, ipd Protein, glycoprotein nmb Protein, glycoprotein (transmembrane) nmb Protein, glycoprotein nmb L homeolog Protein, Gpnmb Protein, GPNMB Protein, gpnmb.L Protein
- Background
- GPNMB, also known as glycoprotein nmb, is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. This protein participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages. It may be involved in growth delay and reduction of metastatic potential. Recombinant human GPNMB protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- Molecular Weight
- 51.8kDa (462aa) 50-70KDa (SDS-PAGE under reducing conditions.)
- NCBI Accession
- NP_002501
- UniProt
- Q14956
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