Osteoactivin Protein (GPNMB) (AA 23-496) (His tag)

Catalog No. ABIN7491429

This Recombinant Osteoactivin protein is produced in HEK-293 Cells.

Quick Overview for Osteoactivin Protein (GPNMB) (AA 23-496) (His tag) (ABIN7491429)

Target: Osteoactivin (GPNMB)

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 23-496

Purification tag / Conjugate: This Osteoactivin protein is labelled with His tag.

Purpose: Recombinant Human GPNMB Protein with C-terminal 6xHis tag

Specificity: GPNMB(Lys23-Met496) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: Osteoactivin (GPNMB)

Alternative Name: GPNMB

Background: HGFIN,NMB,PLCA3
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Molecular Weight: predicted molecular mass of 53.9 kDa after removal of the signal peptide. The apparent molecular mass of GPNMB-His is 70-130 kDa due to glycosylation.

UniProt: Q14956