H2AFJ Protein (His tag)

Catalog No. ABIN2722380

Product Details

Target: H2AFJ (H2A Histone Family, Member J (H2AFJ))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This H2AFJ protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human H2A histone family, member J (H2AFJ), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50 μg (full length, N-term HIS tag, transcript variant 1) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: H2AFJ (H2A Histone Family, Member J (H2AFJ))

Abstract: H2AFJ Products

Synonyms: H2AJ Protein, H2a/j Protein, H2A-IX Protein, H2AFJ Protein, E130307C13 Protein, h2afj Protein, H2A histone family member J Protein, H2A histone family, member J Protein, histone H2A.J Protein, H2A histone family member J L homeolog Protein, H2AFJ Protein, H2afj Protein, LOC103690002 Protein, h2afj.L Protein

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is located on chromosome 12 and encodes a replication-independent histone that is a variant H2A histone. The protein is divergent at the C-terminus compared to the consensus H2A histone family member. This gene also encodes an antimicrobial peptide with antibacterial and antifungal activity.[provided by RefSeq, Oct 2015].

Molecular Weight: 14 kDa

NCBI Accession: NP_808760