HIST1H2BO Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2722791

This Recombinant HIST1H2BO protein is produced in HEK-293 Cells.

Quick Overview for HIST1H2BO Protein (Myc-DYKDDDDK Tag) (ABIN2722791)

Target: HIST1H2BO (Histone Cluster 1, H2bo (HIST1H2BO))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This HIST1H2BO protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Histone cluster 1, H2bo (HIST1H2BO) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HIST1H2BO (Histone Cluster 1, H2bo (HIST1H2BO))

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Molecular Weight: 13.7 kDa

NCBI Accession: NP_003518