IRF5 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2723539

This Recombinant IRF5 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for IRF5 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2723539)

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This IRF5 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Interferon regulatory factor 5 (IRF5), transcript variant 1 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Griesbeck, Ziegler, Laffont, Smith, Chauveau, Tomezsko, Sharei, Kourjian, Porichis, Hart, Palmer, Sirignano, Beisel, Hildebrandt, Cénac, Villani, Diefenbach, Le Gall, Schwartz, Herbeuval, Autran et al.: "Sex Differences in Plasmacytoid Dendritic Cell Levels of IRF5 Drive Higher IFN-α Production in Women. ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 195, Issue 11, pp. 5327-36, (2015) (PubMed).

Target Details

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Background: This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment.

Molecular Weight: 56.5 kDa

NCBI Accession: NP_002191

Pathways: TLR Signaling, Autophagy