KPNA3 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2724405

Product Details

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This KPNA3 protein is labelled with Myc-DYKDDDDK Tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human KPNA3 / Importin alpha-3 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: KPNA3 (Karyopherin alpha 3 (Importin alpha 4) (KPNA3))

Alternative Name: Kpna3,importin alpha-3 (KPNA3 Products)

Synonyms: IPOA4 Protein, si:dz79f24.2 Protein, importin Protein, SRP1 Protein, SRP1gamma Protein, SRP4 Protein, hSRP1 Protein, ipoa4 Protein, srp1 Protein, srp1gamma Protein, srp4 Protein, karyopherin (importin) alpha 3 Protein, karyopherin alpha 3 (importin alpha 4) Protein, karyopherin subunit alpha 3 Protein, karyopherin alpha 3 (importin alpha 4) L homeolog Protein, Kpna3 Protein, kpna3 Protein, KPNA3 Protein, kpna3.L Protein

Background: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 57.6 kDa

NCBI Accession: NP_002258

Pathways: Protein targeting to Nucleus