Midkine Protein (Transcript Variant 1)
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- Target See all Midkine (MDK) Proteins
- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Mouse
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human Midkine (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Determined by its ability to chemoattract human neutrophils using a concentration range of 10-100 ng/ml.
- Top Product
- Discover our top product MDK Protein
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Midkine (Neurite Growth-Promoting Factor 2) (MDK) (Active) protein
MDK Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 23-140) protein (His tag)MDK Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) protein (His-Avi Tag)MDK Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-140) protein (His tag)MDK Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 1-143) protein (GST tag)MDK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 21-143) protein (His tag)Crystallography grade MDK Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Midkine (Neurite Growth-Promoting Factor 2) (MDK) (AA 23-140) protein (His tag)Crystallography grade MDK Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))
- Alternative Name
- Midkine (MDK Products)
- Molecular Weight
- 13.3 kDa
- NCBI Accession
- NP_034914
- Pathways
- RTK Signaling, M Phase, Skeletal Muscle Fiber Development
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