Quick Overview for MITD1 Protein (Myc-DYKDDDDK Tag) (ABIN2726066)
Target
See all MITD1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This MITD1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human MITD1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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MIT, Microtubule Interacting and Transport, Domain Containing 1 (MITD1) (AA 1-249) protein (His tag)
custom-made MITD1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
MIT, Microtubule Interacting and Transport, Domain Containing 1 (MITD1) (AA 1-249) protein (His tag)custom-made MITD1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- MITD1 (MIT, Microtubule Interacting and Transport, Domain Containing 1 (MITD1))
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Alternative Name
- Mitd1
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Background
- Abscission, the separation of daughter cells at the end of cytokinesis, is effected by endosomal sorting complexes required for transport III (ESCRT-III). The protein encoded by this gene functions as a homodimer, with the N-termini binding to a subset of ESCRT-III subunits and the C-termini binding to membranes. The encoded protein regulates ESCRT-III activity and is required for proper cytokinesis. Several transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 29.1 kDa
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NCBI Accession
- NP_620153
Target
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