MRPS15 Protein (His tag)

Catalog No. ABIN2726377

Product Details

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MRPS15 protein is labelled with His tag.

Application: Antibody Production (AbP), Standard (STD)

Characteristics:

• Recombinant human MRPS15 (full length, N-term HIS tag) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the N-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MRPS15 (Mitochondrial Ribosomal Protein S15 (MRPS15))

Alternative Name: Mrps15 (MRPS15 Products)

Synonyms: CG4207 Protein, DmMRPS15 Protein, Dmel\\CG4207 Protein, MRP-S15 Protein, mRpS15 Protein, zgc:92862 Protein, MPR-S15 Protein, RPMS15 Protein, S15mt Protein, 1500003E24Rik Protein, 2410002B11Rik Protein, Mprs15 Protein, CG4207 gene product from transcript CG4207-RA Protein, mitochondrial ribosomal protein S15 Protein, mitochondrial ribosomal protein S15 L homeolog Protein, bonsai Protein, mrps15 Protein, MRPS15 Protein, mrpS15 Protein, mrps15.L Protein, Mrps15 Protein

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S15P family. The encoded protein is more than two times the size of its E. coli counterpart, with the 12S rRNA binding sites conserved. Between human and mouse, the encoded protein is the least conserved among small subunit ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 15q and 19q.

Molecular Weight: 29.8 kDa

NCBI Accession: NP_112570