Neurotrophin 3 Protein (NTF3) (Transcript Variant 2)
Recombinant Neurotrophin 3 protein expressed in Escherichia coli (E. coli).
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Catalog No. ABIN2727203
Quick Overview for Neurotrophin 3 Protein (NTF3) (Transcript Variant 2) (ABIN2727203)
Target
See all Neurotrophin 3 (NTF3) Proteins
Neurotrophin 3 (NTF3)
Protein Type
Recombinant
Biological Activity
Active
Origin
Human
Source
Escherichia coli (E. coli)
Application
Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
Purity
> 95 % as determined by SDS-PAGE and Coomassie blue staining
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Protein Characteristics
- Transcript Variant 2
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Neurotrophin 3 / NTF3 (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.
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Neurotrophin 3 (NTF3) (AA 1-257) protein (GST tag)
NTF3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Neurotrophin 3 (NTF3) (AA 19-128) protein (GST tag)NTF3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Neurotrophin 3 (NTF3)
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Alternative Name
- Neurotrophin 3,ntf3
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Background
- Seems to promote the survival of visceral and proprioceptive sensory neurons. [UniProtKB/Swiss-Prot Function]
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Molecular Weight
- 13.6 kDa
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NCBI Accession
- NP_002518
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Pathways
- RTK Signaling, Neurotrophin Signaling Pathway
Target
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