NUF2 Protein (His tag)
-
- Target See all NUF2 Proteins
- NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This NUF2 protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
-
- Recombinant human NUF2 / CDCA1 (full length, N-term HIS tag, transcript variant 1) protein expressed in E.coli.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product NUF2 Protein
-
-
- Application Notes
-
Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
-
The tag is located at the N-terminal.
- Restrictions
- For Research Use only
-
- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris, pH 8.0, 150 mM NaCl, 10 % glycerol, 1 % Sarkosyl.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
-
- Target
- NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))
- Alternative Name
- Nuf2,cdca1 (NUF2 Products)
- Synonyms
- 2410003C07Rik Protein, AU044112 Protein, C85691 Protein, Cdca1 Protein, NUF2R Protein, CDCA1 Protein, CT106 Protein, zgc:56708 Protein, cdca1 Protein, ct106 Protein, nuf2 Protein, nuf2-a Protein, nuf2-b Protein, nuf2r Protein, xNuf2 Protein, cdca1-B Protein, xnuf2 Protein, NUF2, NDC80 kinetochore complex component Protein, NUF2, NDC80 kinetochore complex component, homolog Protein, spindle pole body protein Nuf2 Protein, NUF2, NDC80 kinetochore complex component L homeolog Protein, NUF2, NDC80 kinetochore complex component S homeolog Protein, Nuf2 Protein, NUF2 Protein, nuf2 Protein, nuf2.L Protein, nuf2.S Protein
- Background
- This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
- Molecular Weight
- 54.1 kDa
- NCBI Accession
- NP_663735
- Pathways
- Maintenance of Protein Location
-