PAK1 Protein (Transcript Variant 1)
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- Target See all PAK1 Proteins
- PAK1 (P21-Activated Kinase 1 (PAK1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- Transcript Variant 1
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- Functional Studies (Func), Antibody Production (AbP), Standard (STD), Protein Interaction (PI)
- Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
- Characteristics
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- Recombinant human PAK1 (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- Purity
- > 90 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- Specific activity was determined as 32,638 pmoles/min/μg, according to the Zlyte assay protocol
- Top Product
- Discover our top product PAK1 Protein
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P21-Activated Kinase 1 (PAK1) (AA 1-545) (Active) protein (GST tag)
PAK1 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
P21-Activated Kinase 1 (PAK1) (AA 248-545) (Active) proteinPAK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS, Func Active
P21-Activated Kinase 1 (PAK1) (AA 1-545) (Active) protein (His-GST)PAK1 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
P21-Activated Kinase 1 (PAK1) (AA 1-447) protein (GST tag)PAK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
P21-Activated Kinase 1 (PAK1) (AA 191-280) protein (GST tag)PAK1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
P21-Activated Kinase 1 (PAK1) (AA 2-545) protein (His tag)Crystallography grade PAK1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
P21-Activated Kinase 1 (PAK1) (AA 2-545) protein (His tag)Crystallography grade PAK1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
P21-Activated Kinase 1 (PAK1) (AA 1-545), (full length) protein (His-SUMO Tag)PAK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10 % glycerol, 5 mM DTT.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PAK1 (P21-Activated Kinase 1 (PAK1))
- Alternative Name
- Pak1 (PAK1 Products)
- Synonyms
- X-PAK1 Protein, XPak1 Protein, pak Protein, pak-1 Protein, pakalpha Protein, PAKalpha Protein, AW045634 Protein, PAK-1 Protein, Paka Protein, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog Protein, p21 (RAC1) activated kinase 1 Protein, p21 protein (Cdc42/Rac)-activated kinase 1 Protein, Serine/threonine-protein kinase pak-1 Protein, pak1.S Protein, PAK1 Protein, Pak1 Protein, pak-1 Protein
- Background
- This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
- Molecular Weight
- 33.6 kDa
- NCBI Accession
- NP_001122092
- Pathways
- MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis
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