PGK1 Protein (Myc-DYKDDDDK Tag)
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- Target See all PGK1 Proteins
- PGK1 (Phosphoglycerate Kinase 1 (PGK1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PGK1 protein is labelled with Myc-DYKDDDDK Tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Phosphoglycerate kinase 1 (PGK1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Purity
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Top Product
- Discover our top product PGK1 Protein
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Phosphoglycerate Kinase 1 (PGK1) (AA 307-417) protein (His-IF2DI Tag)
PGK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) protein (His tag)PGK1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) protein (His tag)PGK1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Phosphoglycerate Kinase 1 (PGK1) (AA 1-417) protein (GST tag)PGK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phosphoglycerate Kinase 1 (PGK1) (AA 321-417) protein (GST tag)PGK1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phosphoglycerate Kinase 1 (PGK1) (AA 2-417) protein (His tag)Crystallography grade PGK1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Phosphoglycerate Kinase 1 (PGK1) (AA 2-417) protein (His tag)Crystallography grade PGK1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- PGK1 (Phosphoglycerate Kinase 1 (PGK1))
- Abstract
- PGK1 Products
- Synonyms
- MIG10 Protein, PGKA Protein, Pgk-1 Protein, Pgk Protein, PGK Protein, wu:fd59b07 Protein, wu:fj36g06 Protein, zgc:56252 Protein, zgc:77899 Protein, PGK1 Protein, phosphoglycerate kinase 1 Protein, pgk2 Protein, pgka Protein, phosphoglycerate kinase 1 Protein, phosphoglycerate kinase 1 L homeolog Protein, PGK1 Protein, Pgk1 Protein, pgk1 Protein, LOC100348124 Protein, pgk1.L Protein
- Background
- The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
- Molecular Weight
- 44.4 kDa
- NCBI Accession
- NP_000282
- Pathways
- Cellular Glucan Metabolic Process
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